EMF Health-effects Research
Effects of different dose microwave radiation on protein components of cultured rabbit lens
Wang KJ, Yao K, Lu DQ
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi, 25(4):208-210, 2007
[Article in Chinese]
OBJECTIVE: To study the effects of different dose microwave radiation on protein components of cultured rabbit lens, and analyze the mechanisms of lens injury caused by microwave radiation.
METHODS: Cultured rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.25, 0.50, 1.00, 2.00, 5.00 mW/cm2 for 8 hours in vitro. The transparency of lens was observed.
Changes of protein concentration were detected after different lens protein components were extracted, including water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP) and sonicated protein (SP).
The influence of microwave radiation on WSP was analyzed using SDS-PAGE electrophoresis and coomassie-blue staining.
RESULTS: Transparency of lens decreased after radiation. There was obvious opacification of lens cortex after 5.00 mW/cm2 microwave radiation for 8 hours. After 1.00, 2.00 and 5.00 mW/cm2 radiation, the percentage of WSP decreased while USP increased obviously. There was no change of ASP.
The percentage of SP decreased when the power of microwave was 5.00 mW/cm2. The low molecular weight protein of WSP decreased while high molecular weight protein increased after microwave radiation.
CONCLUSION: Microwave radiation higher than 1.00 mW/cm2 can affect the proportion of WSP and USP in cultured rabbit lens, and cause changes of lens transparency and refractive power, which leads to lens opacity.