EMF Health-effects Research
Changes in gap junctional intercellular communication in rabbits lens epithelial cells induced by low power density microwave radiation.
Ye J, Yao K, Zeng Q, Lu D
hin Med J (Engl) 115(12):1873-1876, 2002
Ye J, Yao
OBJECTIVE: To demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms.
METHODS: Rabbits' eyes were exposed to 5 mW/cm2 and 10 mW/cm2 power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy.
RESULTS: The GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm2 irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm2 x 3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm2 group.
CONCLUSIONS: Low power densities microwave radiation (5 mW/cm2 and 10 mW/cm2) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm2 or 10 mW/cm2 microwave radiation is cataractogenic.